Effect of Additional Amino Group to Improve the Performance of Immobilized Lipase From Aspergillus niger by Adsorption-Crosslinking Method

Adsorption-crosslinking is one of the immobilization methods to improve reusability lipase. It requires amino groups reduce cross-link risk so that lipase–support interaction increases and attainable. Also, group on support expected increase lipase performance. This study aimed analyze effect addition immobilized Aspergillus niger by adsorption-crosslinking using MP-64 macroporous anion resin XAD-7HP nonionic has been treated with chitosan. The chitosan-coated was characterized Fourier transform infrared spectrometry (FTIR) scanning electron microscope (SEM). Lipase carried out adding 10 ml solution containing 0.75 g resins shaken at 25°C for 150 rpm. Adsorption achieved 4 h, followed cross-linking separately (adding 0.5% (v/v) glutaraldehyde re-reacting 20 min). activity measured titrimetric olive oil emulsion; mixed lipase, emulsion, a buffer (pH 6.5, ionic strength 0.7); incubated 30 min 37°C. amino-functional investigated based loading activity. best 83.79% 29.41 U/g were in coated After four cycles, biodiesel synthesis maintained 70.61% initial yield. These results indicated chitosan as an affordable readily available source could be used modify immobilization.